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Cell Rep. 2013 Jul 25;4(2):352-61. doi: 10.1016/j.celrep.2013.06.036. Epub 2013 Jul 18.

Innate immune-directed NF-κB signaling requires site-specific NEMO ubiquitination.

Author information

1
Department of Pathology, Case Western Reserve School of Medicine, Cleveland, OH 44106, USA.

Abstract

While the I kappa kinase (IKK) scaffolding protein NF-κB essential modulator (NEMO) binds to polyubiquitin chains to transmit inflammatory signals, NEMO itself is also ubiquitinated in response to a variety of inflammatory agonists. Although there have been hints that polyubiquitination of NEMO is essential for avoiding inflammatory disorders, the in vivo physiologic role of NEMO ubiquitination is unknown. In this work, we knock in a NEMO allele in which two major inflammatory agonist-induced ubiquitination sites cannot be ubiquitinated. We show that mice with a nonubiquitinatable NEMO allele display embryonic lethality. Heterozygous females develop inflammatory skin lesions, decreased B cell numbers, and hypercellular spleens. Embryonic lethality can be complemented by mating onto a TNFR1(-/-) background, at the cost of severe steatohepatitis and early mortality, and we also show that NEMO ubiquitination is required for optimal innate immune signaling responses. These findings suggest that NEMO ubiquitination is crucial for NF-κB activity in response to innate immune agonists.

PMID:
23871670
PMCID:
PMC3843603
DOI:
10.1016/j.celrep.2013.06.036
[Indexed for MEDLINE]
Free PMC Article

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