Format

Send to

Choose Destination
PLoS One. 2013 Jul 15;8(7):e68739. doi: 10.1371/journal.pone.0068739. Print 2013.

Assessing the fecal microbiota: an optimized ion torrent 16S rRNA gene-based analysis protocol.

Author information

1
Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Italy.

Abstract

Assessing the distribution of 16S rRNA gene sequences within a biological sample represents the current state-of-the-art for determination of human gut microbiota composition. Advances in dissecting the microbial biodiversity of this ecosystem have very much been dependent on the development of novel high-throughput DNA sequencing technologies, like the Ion Torrent. However, the precise representation of this bacterial community may be affected by the protocols used for DNA extraction as well as by the PCR primers employed in the amplification reaction. Here, we describe an optimized protocol for 16S rRNA gene-based profiling of the fecal microbiota.

PMID:
23869230
PMCID:
PMC3711900
DOI:
10.1371/journal.pone.0068739
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center