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J Hum Reprod Sci. 2013 Jan;6(1):23-6. doi: 10.4103/0974-1208.112375.

Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature?

Author information

1
Department of Reproductive Medicine, Mohak Hi-tech Speciality Laparoscopy and Infertility Centre, Sri Aurobindo Institute of Medical Sciences, Indore, Madhya Pradesh, India.

Abstract

AIM:

To evaluate whether semen processing at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature (RT) by swim-up method.

SETTINGS:

This study was done at tertiary care center attached to Reproductive Medicine Unit and Medical College.

DESIGN:

Prospective pilot study.

PATIENTS:

Normozoospermic men (n = 50) undergoing diagnostic semen analysis.

MATERIALS AND METHODS:

Normozoospermic samples (World Health Organization, 2010 criteria) after analysis was divided into two aliquots (0.5 mL each); one was processed at 37°C and the other at RT by swim-up method. DNA fragmentation of both samples post wash was calculated by acridine orange method.

STATISTICAL ANALYSIS USED:

The values of sperm DNA fragmentation were represented as mean and standard error (mean ± SEM) of the mean. Paired t-test was used for calculating the sperm DNA integrity difference between post wash at RT and 37°C.

RESULTS:

Statistically significant difference was not observed in post wash sperm DNA fragmentation values at 37°C compared to RT.

CONCLUSION:

Our data represents that there was no significant difference in sperm DNA fragmentation values of samples processed at 37°C and at RT. Hence, sperm processing at 37°C does not yield sperm with better DNA integrity compared to centrifugation and processing at RT.

KEYWORDS:

Acridine orange; semen preparation; sperm DNA integrity; temperature

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