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Cell Physiol Biochem. 2013;32(1):64-73. doi: 10.1159/000350125. Epub 2013 Jul 4.

miR-203 suppresses tumor growth and angiogenesis by targeting VEGFA in cervical cancer.

Author information

1
Department of Gynecology and Obstetric Medicine, Shanghai Jiangwan Hospital, Shanghai, P. R. China.

Abstract

BACKGROUND/AIMS:

MicroRNA (miRNA) plays important roles in the development of different cancers. In this study, we investigated the roles and mechanisms of miR-203 in human cervical cancer.

METHODS:

miR-203 expression was detected in cervical cancer tumors and cell lines by qRT-PCR. The methylation status in the promoter region of miR-203 was examined by methylation-specific PCR. The functional effect of miR-203 was determined by both in vitro and in vivo assays.

RESULTS:

miR-203 was frequently down-regulated in cervical cancer tumors and cell lines. This down-regulation of miR-203 was associated with methylation of the miR-203 promoter. Furthermore, miR-203 down-regulated vascular endothelial growth factor alpha (VEGFA) expression by directly targeting its 3'-untranslated region. Functional assays revealed that miR-203 suppressed cervical cancer cell proliferation, tumor growth, and angiogenesis in nude mice, whereas forced expression of VEGFA rescued this inhibitory effect.

CONCLUSION:

Our collective findings indicate that miR-203 functions as a tumor suppressor by targeting VEGFA, resulting in the inhibition of tumor growth and angiogenesis. Thus, miR-203 may be a potential therapeutic target and prognostic marker in cervical cancer.

PMID:
23867971
DOI:
10.1159/000350125
[Indexed for MEDLINE]
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