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Comp Biochem Physiol B Biochem Mol Biol. 2013 Sep;166(1):65-72. doi: 10.1016/j.cbpb.2013.07.004. Epub 2013 Jul 16.

Effects of dietary supplementation of coriander oil, in canola oil diets, on the metabolism of [1-(14)C] 18:3n-3 and [1-(14)C] 18:2n-6 in rainbow trout hepatocytes.

Author information

1
Department of Animal and Poultry Science, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada. kyla.zatti@usask.ca

Abstract

The aim of this study was to investigate the effects of petroselinic acid, found in coriander oil, on the ability of rainbow trout hepatocytes to increase the production of eicosapentaenoic acid (20:5n-3; EPA) and docosahexaenoic acid (22:6n-3; DHA) from [1-(14)C] α-linolenic acid (18:3n-3; ALA) and to reduce the production of arachidonic acid (20:4n-6; ARA) from [1-(14)C] 18:2n-6. Addition of coriander oil increased the production of 22:6n-3, from [1-(14)C] 18:3n-3, at the 0.5 and 1.0% inclusion levels and reduced the conversion of [1-(14)C] 18:2n-6 to 20:4n-6. β-Oxidation was significantly increased at the 1.5% inclusion level for [1-(14)C] 18:2n-6, however β-oxidation for [1-(14)C] 18:3n-3 only showed an increasing trend. Acetate, a main breakdown product of fatty acids (FA) via peroxisomal β-oxidation, decreased three-fold for [1-(14)C] 18:2n-6 and nearly doubled for [1-(14)C] 18:3n-3 when coriander was added at a 1.5% inclusion level. Acyl coenzyme A oxidase (ACO) enzyme activity showed no significant differences between treatments. Relative gene expression of ∆6 desaturase decreased with addition of coriander oil compared to the control. The addition of petroselinic acid via coriander oil to vegetable oil (VO) based diets containing no fishmeal (FM) or fish oil (FO), significantly increased the production of anti-inflammatory precursor 22:6n-3 (P=0.011) and decreased pro-inflammatory precursor 20:4n-6 (P=0.023) in radiolabelled hepatocytes of rainbow trout.

KEYWORDS:

ACO; ALA; ARA; ASP; Acid soluble products; Arachidonic acid; BSA; CP; CPT1; Carnitine palmitoyl transferase I; Coriander oil; DHA; Delta 5 desaturase; Delta 6 desaturase; Docosahexaenoic acid; EDTA; EF1A; EPA; ETIF3; FA; FBS; FFA; FM; FO; HEPES; Hepatocytes; LA; LC-PUFA; Long chain polyunsaturated fatty acids; MG; MUFA; Monoglycerides; Monounsaturated fatty acids; PBS; PL; PUFA; Rainbow trout; SFA; Saturated fatty acids; TAG; TLC; Triacylglyerols; VO; Vegetable oil; acyl coenzyme A oxidase; arachidonic acid; bovine serum albumin; crude protein; docosahexaenoic acid; eicosapentaenoic acid; elongation factor 1a; ethylenediaminetetraacetic acid; eukaryotic translation initiation factor 3; fatty acid; fish oil; fishmeal; foetal bovine serum; free fatty acids; linoleic acid; phenylethylamine and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; phosphate buffer saline; phospholipids; polyunsaturated fatty acids; thin-layer chromatography; α-linolenic acid; ∆5; ∆6

PMID:
23867781
DOI:
10.1016/j.cbpb.2013.07.004
[Indexed for MEDLINE]

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