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Bioresour Technol. 2013 Sep;144:186-93. doi: 10.1016/j.biortech.2013.06.089. Epub 2013 Jun 27.

Recycling cellulase from enzymatic hydrolyzate of acid treated wheat straw by electroultrafiltration.

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National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.


This work explores the feasibility of recycling cellulase by electroultrafiltration (EUF), an ultrafiltration process enhanced by an electric field, to reduce the cost of enzymatic transformation of cellulose. The effect of electric field under different operating conditions (buffer concentration, acid treated wheat straw concentration, current and temperature) on flux during EUF was examined. The results showed that EUF was effective to reduce concentration polarization (CP) and enhance filtration flux in recycling cellulase. The flux improvement by the electric field could be strengthened at low buffer concentration (5 mM) and relatively low temperature (room temperature) and high current (150 mA). The flux for 2% (substrate concentration, w/v) lignocellulosic hydrolyzate increased by a factor of 4.4 at 836 V/m and room temperature, compared to that without electric field. This work shows that under appropriate operating conditions EUF can efficiently recycle cellulase from lignocellulosic hydrolyzate and thus substantially reduce hydrolysis cost.


Cellulase; Concentration polarization; Electroultrafiltration; Lignocellulosic hydrolyzate; Ultrafiltration

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