Format

Send to

Choose Destination
J Virol. 2013 Sep;87(18):10381-4. doi: 10.1128/JVI.00636-13. Epub 2013 Jul 17.

Uncoupling of influenza A virus transcription and replication through mutation of the unpaired adenosine in the viral RNA promoter.

Author information

1
University of Oxford, Sir William Dunn School of Pathology, Oxford, United Kingdom.

Abstract

Transcription and replication of the influenza A virus RNA genome are mediated by the viral RNA polymerase from a promoter consisting of the partially base-paired 3' and 5' termini of viral genome segments. Here we show that transcription and replication can be uncoupled by mutation of an unpaired adenosine in the 5' strand of the promoter. This residue is important for transcription but not replication by being essential for the cap-binding activity of the RNA polymerase.

PMID:
23864616
PMCID:
PMC3753995
DOI:
10.1128/JVI.00636-13
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center