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J Enzyme Inhib Med Chem. 2014 Aug;29(4):485-90. doi: 10.3109/14756366.2013.805756. Epub 2013 Jul 16.

A thermostable trypsin inhibitor with antiproliferative activity from small pinto beans.

Author information

1
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories , Hong Kong , China and.

Abstract

Small pinto bean is a cultivar of Phaseolus vulgaris. It produces a 16-kDa trypsin inhibitor that could be purified using anion exchange and size chromatography. Q-Sepharose, Mono Q and Superdex 75 columns were employed for the isolation process. Small pinto bean trypsin inhibitor demonstrated moderate pH stability (pH 2-10) and marked heat stability, with its trypsin inhibitory activity largely retained after exposure to 100 °C for half an hour. The activity was abolished in the presence of dithiothreitol, in a dose-dependent manner, implying that disulfide bonds in small pinto bean trypsin inhibitor are crucial for the activity. The trypsin inhibitor showed a blocked N-terminus. The trypsin inhibitor only slightly inhibited the viability of breast cancer MCF7 and hepatoma HepG2 cells at 125 μM.

KEYWORDS:

Bowman--Birk; Phaseolus vulgaris; dithiothreitol; leguminous

PMID:
23859150
DOI:
10.3109/14756366.2013.805756
[Indexed for MEDLINE]

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