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Circ Res. 2013 Sep 13;113(7):856-62. doi: 10.1161/CIRCRESAHA.113.302035. Epub 2013 Jul 15.

Jmjd3 controls mesodermal and cardiovascular differentiation of embryonic stem cells.

Author information

1
From the Institute of Cardiovascular Regeneration, Centre for Molecular Medicine, University of Frankfurt, Frankfurt, Germany.

Abstract

RATIONALE:

The developmental role of the H3K27 demethylases Jmjd3, especially its epigenetic regulation at target genes in response to upstream developmental signaling, is unclear.

OBJECTIVE:

To determine the role of Jmjd3 during mesoderm and cardiovascular lineage commitment.

METHODS AND RESULTS:

Ablation of Jmjd3 in mouse embryonic stem cells does not affect the maintenance of pluripotency and self-renewal but compromised mesoderm and subsequent endothelial and cardiac differentiation. Jmjd3 reduces H3K27me3 marks at the Brachyury promoter and facilitates the recruitment of β-catenin, which is critical for Wnt signal-induced mesoderm differentiation.

CONCLUSIONS:

These data demonstrate that Jmjd3 is required for mesoderm differentiation and cardiovascular lineage commitment.

KEYWORDS:

Brachyury protein; Jmjd3 protein, mouse; Wnt signaling pathway; embryonic stem cells; epigenomics; mesoderm

Comment in

PMID:
23856522
DOI:
10.1161/CIRCRESAHA.113.302035
[Indexed for MEDLINE]

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