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Nat Methods. 2013 Sep;10(9):857-60. doi: 10.1038/nmeth.2563. Epub 2013 Jul 14.

In situ sequencing for RNA analysis in preserved tissue and cells.

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1
Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.

Abstract

Tissue gene expression profiling is performed on homogenates or on populations of isolated single cells to resolve molecular states of different cell types. In both approaches, histological context is lost. We have developed an in situ sequencing method for parallel targeted analysis of short RNA fragments in morphologically preserved cells and tissue. We demonstrate in situ sequencing of point mutations and multiplexed gene expression profiling in human breast cancer tissue sections.

PMID:
23852452
DOI:
10.1038/nmeth.2563
[Indexed for MEDLINE]
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