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Hum Vaccin Immunother. 2013 Oct;9(10):2157-64. doi: 10.4161/hv.25656. Epub 2013 Jul 12.

Potent monoclonal antibodies against Clostridium difficile toxin A elicited by DNA immunization.

Author information

1
Jiangsu Province Key Laboratory in Infectious Diseases; The First Affiliated Hospital of Nanjing Medical University; Nanjing, P.R. China; Department of Infectious Diseases; The First Affiliated Hospital of Nanjing Medical University; Nanjing, P.R. China; China-US Vaccine Research Center; The First Affiliated Hospital of Nanjing Medical University; Nanjing, P.R. China.
2
Jiangsu Haiyuan Protein Biotech Co. Ltd.; Taizhou, P.R. China.
3
ICDC, Chinese Center for Disease Control and Prevention, Beijing, P.R. China.
4
China-US Vaccine Research Center; The First Affiliated Hospital of Nanjing Medical University; Nanjing, P.R. China; Department of Medicine; University of Massachusetts Medical School; Worcester, MA USA.

Abstract

Recent studies have demonstrated that DNA immunization is effective in eliciting antigen-specific antibody responses against a wide range of infectious disease targets. The polyclonal antibodies elicited by DNA vaccination exhibit high sensitivity to conformational epitopes and high avidity. However, there have been limited reports in literature on the production of monoclonal antibodies (mAb) by DNA immunization. Here, by using Clostridium difficile (C. diff) toxin A as a model antigen, we demonstrated that DNA immunization was effective in producing a panel of mAb that are protective against toxin A challenge and can also be used as sensitive reagents to detect toxin A from various testing samples. The immunoglobulin (Ig) gene usage for such mAb was also investigated. Further studies should be conducted to fully establish DNA immunization as a unique platform to produce mAb in various hosts.

KEYWORDS:

C. difftoxin; DNA immunization; immunoglobulin genes; monoclonal antibody; toxin A

PMID:
23851482
PMCID:
PMC3906400
DOI:
10.4161/hv.25656
[Indexed for MEDLINE]
Free PMC Article

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