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Bioengineered. 2013 Sep-Oct;4(5):313-21. doi: 10.4161/bioe.24159. Epub 2013 Aug 29.

Identification and characterization of an oleate hydratase-encoding gene from Bifidobacterium breve.

Author information

1
Department of Microbiology; University College Cork; Cork, Ireland; Alimentary Pharmabiotic Centre; University College Cork; Cork, Ireland; Teagasc Research Centre Moorepark; Fermoy; Cork, Ireland; Department of Plant Biochemistry; Georg-August-University; Goettingen, Germany.

Abstract

Bifidobacteria are common commensals of the mammalian gastrointestinal tract. Previous studies have suggested that a bifidobacterial myosin cross reactive antigen (MCRA) protein plays a role in bacterial stress tolerance, while this protein has also been linked to the biosynthesis of conjugated linoleic acid (CLA) in bifidobacteria. In order to increase our understanding on the role of MCRA in bifidobacteria we created and analyzed an insertion mutant of the MCRA-encoding gene of B. breve NCFB 2258. Our results demonstrate that the MCRA protein of B. breve NCFB 2258 does not appear to play a role in CLA production, yet is an oleate hydratase, which contributes to bifidobacterial solvent stress protection.

KEYWORDS:

bifidobacteria; conjugated linoleic acid; hydratase; myosin cross reactive antigen; probiotic

PMID:
23851389
PMCID:
PMC3813531
DOI:
10.4161/bioe.24159
[Indexed for MEDLINE]
Free PMC Article

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