A novel Bacillus thuringiensis Cry-like protein from a rare filamentous strain promotes crystal localization within the exosporium

Appl Environ Microbiol. 2013 Sep;79(18):5774-6. doi: 10.1128/AEM.01206-13. Epub 2013 Jul 12.

Abstract

Mutation of a novel cry-like gene (cry256) from Bacillus thuringiensis resulted in a protein crystal, normally located within the spore's exosporium, being found predominately outside the exosporium. The cry256 gene codes for a 3-domain Cry-like protein that does not correspond to any of the known Cry protein holotypes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus thuringiensis / genetics
  • Bacillus thuringiensis / metabolism*
  • Bacillus thuringiensis Toxins
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Endotoxins / genetics
  • Endotoxins / metabolism*
  • Hemolysin Proteins / genetics
  • Hemolysin Proteins / metabolism*
  • Molecular Sequence Data
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Spores, Bacterial / genetics
  • Spores, Bacterial / metabolism*

Substances

  • Bacillus thuringiensis Toxins
  • Bacterial Proteins
  • DNA, Bacterial
  • Endotoxins
  • Hemolysin Proteins
  • insecticidal crystal protein, Bacillus Thuringiensis

Associated data

  • GENBANK/JQ670887