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J Clin Microbiol. 2013 Sep;51(9):3123-6. doi: 10.1128/JCM.01720-13. Epub 2013 Jul 12.

Interlaboratory comparison of intact-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry results for identification and differentiation of Brucella spp.

Author information

1
Friedrich-Loeffler-Institut, Institute of Molecular Biology, Greifswald-Insel Riems, Germany.

Abstract

Classical microbiological diagnosis of human brucellosis is time-consuming, hazardous, and subject to variable interpretation. Intact-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for the routine identification of Brucella spp. Analysis of mass peak patterns allowed accurate identification to the genus level. However, statistical models based on peak intensities were needed for definite species differentiation. Interlaboratory comparison confirmed the reproducibility of the results.

PMID:
23850950
PMCID:
PMC3754649
DOI:
10.1128/JCM.01720-13
[Indexed for MEDLINE]
Free PMC Article

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