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Trends Microbiol. 2013 Aug;21(8):397-404. doi: 10.1016/j.tim.2013.06.004. Epub 2013 Jul 11.

Catching HIV 'in the act' with 3D electron microscopy.

Author information

1
Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Abstract

The development of a safe, effective vaccine to prevent HIV infection is a key step for controlling the disease on a global scale. However, many aspects of HIV biology make vaccine design problematic, including the sequence diversity and structural variability of the surface envelope glycoproteins and the poor accessibility of neutralization-sensitive epitopes on the virus. In this review, we discuss recent progress in understanding HIV in a structural context using emerging tools in 3D electron microscopy, and outline how some of these advances could be important for a better understanding of mechanisms of viral entry and for vaccine design.

KEYWORDS:

cryo-electron tomography; focused ion beam scanning electron microscopy (FIB-SEM); ion abrasion scanning electron microscopy (IA-SEM); vaccine design; virus–cell interaction

PMID:
23850373
PMCID:
PMC3773172
DOI:
10.1016/j.tim.2013.06.004
[Indexed for MEDLINE]
Free PMC Article

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