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Nat Protoc. 2013 Aug;8(8):1513-24. doi: 10.1038/nprot.2013.090. Epub 2013 Jul 11.

Generation of eggs from mouse embryonic stem cells and induced pluripotent stem cells.

Author information

1
Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan. khayashi@anat2.med.kyoto-u.ac.jp

Abstract

Oogenesis is an integrated process through which an egg acquires the potential for totipotency, a fundamental condition for creating new individuals. Reconstitution of oogenesis in a culture that generates eggs with proper function from pluripotent stem cells (PSCs) is therefore one of the key goals in basic biology as well as in reproductive medicine. Here we describe a stepwise protocol for the generation of eggs from mouse PSCs, such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). ESCs and iPSCs are first induced into primordial germ cell-like cells (PGCLCs) that are in turn aggregated with somatic cells of female embryonic gonads, the precursors for adult ovaries. Induction of PGCLCs followed by aggregation with the somatic cells takes up to 8 d. The aggregations are then transplanted under the ovarian bursa, in which PGCLCs grow into germinal vesicle (GV) oocytes in ∼1 month. The PGCLC-derived GV oocytes can be matured into eggs in 1 d by in vitro maturation (IVM), and they can be fertilized with spermatozoa by in vitro fertilization (IVF) to obtain healthy and fertile offspring. This method provides an initial step toward reconstitution of the entire process of oogenesis in vitro.

PMID:
23845963
DOI:
10.1038/nprot.2013.090
[Indexed for MEDLINE]

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