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J Chromatogr Sci. 2014 Aug;52(7):725-9. doi: 10.1093/chromsci/bmt102. Epub 2013 Jul 11.

Simultaneous determination of five phytohormones in mungbean sprouts of China by micellar electrokinetic chromatography.

Author information

1
College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, People's Republic of China.
2
College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, People's Republic of China lvyunkai@hbu.edu.cn.

Abstract

A simple and rapid micellar electrokinetic chromatography method was developed for simultaneous determination of indole-3-acetic acid, indole-3-butyric acid, gibberellic acid, abscisic acid and naphthylacetic acid in mungbean sprouts for monitoring plant growth and development. The effects of several parameters related to the separation and determination were investigated in detail. The analysis was carried out using 10 mM borax, 10 mM sodium dihydrogen phosphate, 90 mM sodium dodecyl sulfate and 5% acetonitrile as running buffer (pH 9.0). Under optimum conditions, the method demonstrated good performance concerning linearity (r, 0.9954-0.9991), precision (0.77-4.97%), the method limit of detection (LOD) and the method limit of quantitation (LOQ) (LOD, 0.011-0.177 mg/kg; LOQ, 0.035-0.590 mg/kg) and accuracy (83.62-102.56%). The results confirmed that the method is rapid, convenient and of low cost for the determination of the phytohormones.

PMID:
23845886
DOI:
10.1093/chromsci/bmt102
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