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PLoS One. 2013 Jul 2;8(7):e67308. doi: 10.1371/journal.pone.0067308. Print 2013.

Development and characterization of a chemically defined food for Drosophila.

Author information

1
Department of Developmental Biology, Washington University School of Medicine, St. Louis, Missouri, USA.

Abstract

Diet can affect a spectrum of biological processes ranging from behavior to cellular metabolism. Yet, the precise role of an individual dietary constituent can be a difficult variable to isolate experimentally. A chemically defined food (CDF) permits the systematic evaluation of individual macro- and micronutrients. In addition, CDF facilitates the direct comparison of data obtained independently from different laboratories. Here, we report the development and characterization of a CDF for Drosophila. We show that CDF can support the long-term culture of laboratory strains and demonstrate that this formulation has utility in isolating macronutrient from caloric density requirements in studies of development, longevity and reproduction.

PMID:
23844001
PMCID:
PMC3699577
DOI:
10.1371/journal.pone.0067308
[Indexed for MEDLINE]
Free PMC Article

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