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J Cell Sci. 2013 Sep 15;126(Pt 18):4099-107. doi: 10.1242/jcs.120295. Epub 2013 Jul 10.

The role of integrin-linked kinase in the molecular architecture of focal adhesions.

Author information

1
Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer-Sheva 84120, Israel.

Abstract

Integrin-mediated focal adhesions (FAs) are large, multi-protein complexes that link the actin cytoskeleton to the extracellular matrix and take part in adhesion-mediated signaling. These adhesions are highly complex and diverse at the molecular level; thus, assigning particular structural or signaling functions to specific components is highly challenging. Here, we combined functional, structural and biophysical approaches to assess the role of a major FA component, namely, integrin-linked kinase (ILK), in adhesion formation. We show here that ILK plays a key role in the formation of focal complexes, early forms of integrin adhesions, and confirm its involvement in the assembly of fibronectin-bound fibrillar adhesions. Examination of ILK-null fibroblasts by cryo-electron tomography pointed to major structural changes in their FAs, manifested as disarray of the associated actin filaments and an increase in the packing density of FA-related particles. Interestingly, adhesion of the mutant cells to the substrate required a higher ligand density than in control cells. These data indicate that ILK has a key role in integrin adhesion assembly and sub-structure, and in the regulation of the FA-associated cytoskeleton.

KEYWORDS:

Cell adhesion; Correlated microscopy; Cryo-electron tomography; Focal adhesion; Integrin; Integrin-linked kinase

PMID:
23843624
DOI:
10.1242/jcs.120295
[Indexed for MEDLINE]
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