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Toxicol Ind Health. 2015 Dec;31(12):1334-40. doi: 10.1177/0748233713491811. Epub 2013 Jul 10.

The chemoprotective effects of L-carnitine against genotoxicity induced by diazinon in rat blood lymphocyte.

Author information

  • 1Pharmaceutical Sciences Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Islamic Republic of Iran.
  • 2Pharmaceutical Sciences Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Islamic Republic of Iran mslamuk@yahoo.com.
  • 3Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
  • 4Department of Occupational Health, Faculty of Health, North Khorasan University of Medical Sciences, Bojnurd, Islamic Republic of Iran.

Abstract

The purpose of this study was to assess the preventive effects of L-carnitine (LC) against DNA damage induced by diazinon (DZN) in rat blood lymphocytes. Animals were concurrently administered intraperitoneally with DZN in proper solvent (20 mg/kg body weight (b.w.)) and LC at three different doses (50, 100, and 150 mg/kg b.w.) for 30 consecutive days. The positive control group received DZN at the same dose without LC. Twenty-four hour after last injection, 0.5 ml blood of each rat was received and cultured in culture medium for 44 h. The lymphocyte cultures were mitogenically stimulated with cytochalasin B for the evaluation of the number of micronuclei (MNs) in cytokinesis-blocked binucleated cells. Incubation of lymphocytes with DZN induced additional genotoxicity and was shown by increase in MNs frequency in rat lymphocytes. LC at all doses had a protective effect and significantly reduced the MNs frequency in cultured lymphocytes (p < 0.0001-p < 0.05). The maximum effect was observed at 150 mg/kg that reduced the frequency of MN from 12.78 ± 0.24% for DZN group to 5.61 ± 0.17%. Our study revealed that LC has a potent antigenotoxic effect against DZN-induced toxicity in rats, which may be due to the scavenging of free radicals and increased antioxidant status. Since LC is a natural compound and is being safe, it is recommended as a daily supplement for body defense against side effects induced by chemical hazardous agents.

KEYWORDS:

Chemoprotective; diazinon; genotoxicity; l-carnitine; micronucleus assay; rat blood lymphocyte

PMID:
23843223
DOI:
10.1177/0748233713491811
[PubMed - indexed for MEDLINE]
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