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Chem Commun (Camb). 2013 Aug 21;49(65):7243-5. doi: 10.1039/c3cc43224f. Epub 2013 Jul 10.

Backbone-modified molecular beacons for highly sensitive and selective detection of microRNAs based on duplex specific nuclease signal amplification.

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1
The Key Laboratory of Analytical Science, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

Abstract

Based on backbone-modified molecular beacons and duplex-specific nuclease, we have developed a target recycling amplification method for highly sensitive and selective miRNA detection. The combination of a low fluorescence background of 2-OMe-RNA modified MB and nuclease-assisted signal amplification leads to ultrahigh assay sensitivity, and the powerful discriminating ability of MB enables the differentiation of highly similar miRNAs with one-base difference, both of which are of great significance to miRNA detection.

PMID:
23842896
DOI:
10.1039/c3cc43224f
[Indexed for MEDLINE]
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