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J Membr Biol. 2013 Aug;246(8):627-32. doi: 10.1007/s00232-013-9576-1. Epub 2013 Jul 6.

Morphological changes of proteolipid giant unilamellar vesicles affected by Macrovipera lebetina obtusa venom visualized with fluorescence microscope.

Author information

1
Laboratory of Toxicology, Institute of Physiology, NAS RA, Yerevan, Armenia. ghazaryan-n@rambler.ru

Abstract

As a rule, zootoxins are complex and biologically active, and therefore the greater part of zootoxins is subjected to biotransformation and interacts with biological membranes. In this case, the interaction of different venom components with the membranes is not always the same. The present study shows how the giant unilamellar vesicles (GUV) from bovine brain proteolipids interact with Macrovipera lebetina obtusa venom. GUV (mean diameter 30 μm) were formed by the electroformation method. We used 8-anilino-1-naphthalenesulfonic acid and pyrene as fluorescence probes, which allowed us to quantify the fluidity changes in the membrane by measuring the fluorescence intensity.

PMID:
23831955
DOI:
10.1007/s00232-013-9576-1
[Indexed for MEDLINE]

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