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Nat Chem Biol. 2013 Aug;9(8):491-3. doi: 10.1038/nchembio.1289. Epub 2013 Jun 30.

A cell wall recycling shortcut that bypasses peptidoglycan de novo biosynthesis.

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1
Department of Biology, Konstanz Research School Chemical Biology, University of Konstanz, Konstanz, Germany.

Abstract

We report a salvage pathway in Gram-negative bacteria that bypasses de novo biosynthesis of UDP N-acetylmuramic acid (UDP-MurNAc), the first committed peptidoglycan precursor, and thus provides a rationale for intrinsic fosfomycin resistance. The anomeric sugar kinase AmgK and the MurNAc α-1-phosphate uridylyl transferase MurU, defining this new cell wall sugar-recycling route in Pseudomonas putida, were characterized and engineered into Escherichia coli, channeling external MurNAc directly to peptidoglycan biosynthesis.

PMID:
23831760
DOI:
10.1038/nchembio.1289
[Indexed for MEDLINE]
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