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Int J Biol Macromol. 2013 Oct;61:127-34. doi: 10.1016/j.ijbiomac.2013.06.042. Epub 2013 Jul 2.

Optimization of polysaccharides from Lycium ruthenicum fruit using RSM and its anti-oxidant activity.

Author information

1
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.

Abstract

Dynamic microwave-assisted extraction (DMAE) technique was employed for the extraction of polysaccharides from Lycium ruthenicum (LRP). The extracting parameters were optimized by using three-variable-three-level Box-Behnken design and response surface methodology (RSM) based on the single-factor experiments. RSM analysis indicated good correspondence between experimental and predicted values. The optimum extraction parameters for the yield of polysaccharide were ratio of water to raw material 31.5 mL/g, extracting time 25.8 min and microwave power 544.0 W. Polysaccharide was analyzed by chemical methods and Fourier-transform infrared (FT-IR). The antioxidant activities of LRP were investigated including scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide and free radicals of superoxide anion in vitro. The results of antioxidant activity exhibited LRP had the potential to be explored as novel natural antioxidant for using in functional foods or medicine.

KEYWORDS:

Antioxidant activity; Lycium ruthenicum Murr.; Polysaccharides

PMID:
23831533
DOI:
10.1016/j.ijbiomac.2013.06.042
[Indexed for MEDLINE]

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