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Methods Enzymol. 2013;527:221-37. doi: 10.1016/B978-0-12-405882-8.00012-X.

Analysis of environmental stress in plants with the aid of marker genes for H2O2 responses.

Author information

1
The United Graduate School of Agricultural Sciences, Gifu University, Gifu, Japan.

Abstract

Hydrogen peroxide acts as a signaling molecule mediating the acquisition of tolerance to both biotic and abiotic stresses. Identification of marker genes for H2O2 response could help to intercept the signaling network of stress response of plants. Here, we describe application of marker genes for H2O2 responses to monitoring several abiotic stress responses. Arabidopsis plants were treated with UV-B, high light, and cold stresses, where involvement of H2O2-mediated signaling is known or suggested. Monitoring of these stress responses with molecular markers using quantitative real-time RT-PCR can detect landmark events in the sequential stress responses. These methods can be used for analysis of mutants and transgenic plants to examine natural H2O2 responses that are involved in environmental adaptation.

KEYWORDS:

Arabidopsis; Cold; H(2)O(2); High light; Marker gene; Real-time qRT-PCR; UV-B

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