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Anal Chim Acta. 2013 Jul 17;787:203-10. doi: 10.1016/j.aca.2013.05.024. Epub 2013 May 20.

A label-free electrochemical biosensor based on a DNA aptamer against codeine.

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1
Chemistry and Chemical Engineering College, Yunnan Normal University, Kunming 650092, China.

Abstract

In order to develop a sensor for opium alkaloid codeine detection, DNA aptamers against codeine were generated by SELEX (systematic evolution of ligands by exponential enrichment) technique. An aptamer HL7-14, which is a 37-mer sequence with Kd values of 0.91 μM, was optimized by the truncation-mutation assay. The specificity investigation shows that HL7-14 exhibits high specificity to codeine over morphine, and almost cannot bind to other small molecule. With this new selected aptamer, a novel electrochemical label-free codeine aptamer biosensor based on Au-mesoporous silica nanoparticles (Au-MSN) as immobilized substrate has been proposed using [Fe(CN)6](3-/4-) as electroactive redox probe. The linear range covered from 10 pM to 100 nM with correlation coefficient of 0.9979 and the detection limit was 3 pM. Our study demonstrates that the biosensor has good specificity, stability and well regeneration. It can be used to detect codeine.

KEYWORDS:

Aptamer; Au-mesoporous silica nanoparticles; Codeine; Electrochemical aptasensor; Systematic evolution of ligands by exponential enrichment

PMID:
23830440
DOI:
10.1016/j.aca.2013.05.024
[Indexed for MEDLINE]
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