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J Chromatogr Sci. 2014 Aug;52(7):641-5. doi: 10.1093/chromsci/bmt093. Epub 2013 Jul 4.

Simultaneous HPLC quantitative analysis of active compounds in leaves of Moringa oleifera Lam.

Author information

1
Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudthaya Road, Ratchathevi, Bangkok, Thailand 10400.
2
Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudthaya Road, Ratchathevi, Bangkok, Thailand 10400 wandee.gri@mahidol.ac.th wandeegrit@yahoo.co.th.

Abstract

Moringa oleifera Lam. has been used as a traditional medicine for the treatment of numerous diseases. A simultaneous high-performance liquid chromatography (HPLC) analysis was developed and validated for the determination of the contents of crypto-chlorogenic acid, isoquercetin and astragalin, the primary antioxidative compounds, in M. oleifera leaves. HPLC analysis was successfully conducted by using a Hypersil BDS C18 column, eluted with a gradient of methanol-1% acetic acid with a flow rate of 1 mL/min, and detected at 334 nm. Parameters for the validation included linearity, precision, accuracy and limits of detection and quantitation. The developed HPLC method was precise, with relative standard deviation < 2%. The recovery values of crypto-chlorogenic acid, isoquercetin and astragalin in M. oleifera leaf extracts were 98.50, 98.47 and 98.59%, respectively. The average contents of these compounds in the dried ethanolic extracts of the leaves of M. oleifera collected from different regions of Thailand were 0.081, 0.120 and 0.153% (w/w), respectively. The developed HPLC method was appropriate and practical for the simultaneous analysis of crypto-chlorogenic acid, isoquercetin and astragalin in the leaf extract of M. oleifera. This work is valuable as guidance for the standardization of the leaf extracts and pharmaceutical products of M. oleifera.

PMID:
23828911
DOI:
10.1093/chromsci/bmt093
[Indexed for MEDLINE]

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