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Parasitol Res. 2013 Sep;112(9):3229-38. doi: 10.1007/s00436-013-3500-6. Epub 2013 Jul 5.

An antigenic recombinant serine protease from Trichinella spiralis induces protective immunity in BALB/c mice.

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1
Sun Yat-sen University Zhongshan School of Medicine, Key Laboratory of Tropical Disease Control (Sun Yat-Sen University), Ministry of Education, 74 Zhongshan 2nd Road, Guangzhou, 510080, China.

Abstract

In this study, we report the cloning and characterization of a cDNA encoding a Trichinella serine protease gene (TspSP-1.3) from GenBank. The recombinant TspSP-1.3 protein (rTspSP-1.3) was expressed in an Escherichia coli expression system and purified with Ni-affinity chromatography. Real-time quantitative PCR analysis revealed that TspSP-1.3 was expressed at significantly higher levels in muscle larvae and adult worms than in newborn larvae. TspSP-1.3 was detected in excretory-secretory proteins of Trichinella spiralis with western blotting. Immunization with the rTspSP-1.3 antigen induced humoral immune responses, which manifested as elevated specific anti-rTspSP-1.3 IgG and IgE antibodies and a mixed Th1/Th2 response. To determine whether purified rTspSP-1.3 had good antigenicity and could be a vaccine candidate for the control of T. spiralis infection, we immunized BALB/c mice with rTspSP-1.3 and subsequently challenged the mice with T. spiralis larvae. The results showed that mice vaccinated with rTspSP-1.3 exhibited an average reduction in the muscle larvae burden of 39 % relative to the control group. These results suggest that TspSP-1.3 could be a novel vaccine candidate for controlling Trichinella infection.

PMID:
23828191
DOI:
10.1007/s00436-013-3500-6
[Indexed for MEDLINE]
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