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Int J Ophthalmol. 2013 Jun 18;6(3):280-5. doi: 10.3980/j.issn.2222-3959.2013.03.05. Print 2013.

Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells.

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1
Department of Ophthalmology, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.

Abstract

AIM:

To explore the potential mechanism of molecular hydrogen in the regulation of miRNA expression and signal-modulating activities.

METHODS:

Retinal microglia cells were activated by Lipopolysaccharides (LPS) and then treated with hydrogen-saturated medium or normal medium without hydrogen. qRT-PCR was used to detect the expression difference in miR-9, miR-21 and miR-199 between these two groups. Moreover, the expression of LPS-induced signaling proteins, including Myd88, IKK-β, NF-κB, and PDCD4, were detected by Western blotting.

RESULTS:

The results demonstrated a marked down-regulation of miR-9 and miR-21 and up-regulation of miR-199 by hydrogen treatment; the expression of Myd88 and IKK-β was decreased after hydrogen treatment, whereas PDCD4 was increased, and there was no significant change in NF-κB expression.

CONCLUSION:

The results in the present study indicate that miR-9, miR-199 and miR-21 play an important role in the anti-inflammatory regulation of LPS-activated microglia cells by molecular hydrogen, which will help to explain the protective mechanism of molecular hydrogen against inflammatory injury.

KEYWORDS:

Lipopolysaccharides; Toll-like receptor 4; hydrogen; miR-199; miR-21; miR-9

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