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Methods Mol Biol. 2013;1031:71-6. doi: 10.1007/978-1-62703-481-4_9.

Bone marrow-derived dendritic cells.

Author information

1
RTI International, Research Triangle Park, Durham, NC, USA.

Abstract

While much is understood about dendritic cells and their role in the immune system, the study of these cells is critical to gain a more complete understanding of their function. Dendritic cell isolation from mouse body tissues can be difficult and the number of cells isolated small. This protocol describes the growth of large number of dendritic cells from the culture of mouse bone marrow cells. The dendritic cells grown in culture facilitate experiments that may require large number of dendritic cells without great expense or use of large number of mice.

PMID:
23824889
DOI:
10.1007/978-1-62703-481-4_9
[Indexed for MEDLINE]

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