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Curr Protoc Mol Biol. 2013 Jul;Chapter 4:Unit 4.17. doi: 10.1002/0471142727.mb0417s103.

Quantification of microRNA expression with next-generation sequencing.

Author information

1
Department of Genetics, Harvard Medical School, Boston, MA, USA.

Abstract

Rapid advancement of next-generation sequencing technologies has made it possible to study expression profiles of microRNAs (miRNAs) comprehensively and efficiently. Multiplexing miRNA libraries by barcoding can significantly reduce sequencing cost per sample without compromising library quality. This unit provides a step-by-step protocol for isolating miRNAs and constructing multiplexed miRNA libraries. Also described is a custom computational pipeline for analyzing the multiplexed miRNA library sequencing reads generated by Illumina-based technology.

PMID:
23821442
PMCID:
PMC4138881
DOI:
10.1002/0471142727.mb0417s103
[Indexed for MEDLINE]
Free PMC Article

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