Format

Send to

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2013;1030:119-27. doi: 10.1007/978-1-62703-484-5_10.

Expression and purification of hepatitis C virus protease from clinical samples.

Author information

1
Janssen Infectious Diseases-Diagnostics BVBA, Johnson & Johnson Corporation, Beerse, Belgium.

Abstract

This chapter describes the procedures for production of recombinant hepatitis C virus (HCV) NS3 protease from clinical samples, which can be used in the biochemical assays to assess the impact of different drug-resistant mutations in the NS3 protein from patients under therapy of protease inhibitors. It gives the details of expression and purification of NS3 protease using the pCold vectors that contains a promoter derived from the cold-shock genes to drive the expression of NS3 protein. This robust protocol enables a medium-throughput production of HCV NS3 proteins of all genotypes and sequences derived from patient specimen.

PMID:
23821264
DOI:
10.1007/978-1-62703-484-5_10
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Support Center