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Mol Cells. 2013 Jul;36(1):62-8. doi: 10.1007/s10059-013-0044-7. Epub 2013 May 30.

MicroRNA-409-3p inhibits migration and invasion of bladder cancer cells via targeting c-Met.

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1
Department of Urology, First Affiliated Hospital, Zhejiang University, Qingchun Road 79, Hangzhou, 310003, Zhejiang Province, China.

Abstract

There is increasing evidence suggesting that dysregulation of certain microRNAs (miRNAs) may contribute to tumor progression and metastasis. Previous studies have shown that miR-409-3p is dysregulated in some malignancies, but its role in bladder cancer is still unknown. Here, we find that miR-409-3p is down-regulated in human bladder cancer tissues and cell lines. Enforced expression of miR-409-3p in bladder cancer cells significantly reduced their migration and invasion without affecting cell viability. Bioinformatics analysis identified the pro-metastatic gene c-Met as a potential miR-409-3p target. Further studies indicated that miR-409-3p suppressed the expression of c-Met by binding to its 3'-untranslated region. Silencing of c-Met by small interfering RNAs phenocopied the effects of miR-409-3p overexpression, whereas restoration of c-Met in bladder cancer cells bladder cancer cells overexpressing miR-409-3p, partially reversed the suppressive effects of miR-409-3p. We further showed that MMP2 and MMP9 may be downstream effector proteins of miR-409-3p. These findings indicate that miR-409-3p could be a potential tumor suppressor in bladder cancer.

PMID:
23820886
PMCID:
PMC3887926
DOI:
10.1007/s10059-013-0044-7
[Indexed for MEDLINE]
Free PMC Article
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