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Nat Methods. 2013 Aug;10(8):741-3. doi: 10.1038/nmeth.2532. Epub 2013 Jun 30.

Heritable genome editing in C. elegans via a CRISPR-Cas9 system.

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1
Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.

Abstract

We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.

PMID:
23817069
PMCID:
PMC3822328
DOI:
10.1038/nmeth.2532
[Indexed for MEDLINE]
Free PMC Article
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