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Nat Methods. 2013 Aug;10(8):759-61. doi: 10.1038/nmeth.2533. Epub 2013 Jun 30.

Engineered nanostructured β-sheet peptides protect membrane proteins.

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1
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA.

Abstract

We designed β-strand peptides that stabilize integral membrane proteins (IMPs). β-strand peptides self-assemble in solution as filaments and become restructured upon association with IMPs; resulting IMP-β-strand peptide complexes resisted aggregation when diluted in detergent-free buffer and were visible as stable, single particles with low detergent background in electron micrographs. β-strand peptides enabled clear visualization of flexible conformations in the highly dynamic ATP-binding cassette (ABC) transporter MsbA.

PMID:
23817067
PMCID:
PMC3753066
DOI:
10.1038/nmeth.2533
[Indexed for MEDLINE]
Free PMC Article
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