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J Chromatogr Sci. 2014 Aug;52(7):617-23. doi: 10.1093/chromsci/bmt086. Epub 2013 Jun 27.

HPLC-DAD determination of seven antioxidants and caffeine in different phytopharmaceuticals.

Author information

  • 1Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
  • 2Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt randa_mousa@pharm.suez.edu.eg.
  • 3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sinai University, El Arish, North Sinai, Egypt Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Umm Al-Qura University, Makkah, Kingdom of Saudi Arabia.
  • 4Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.

Abstract

A high-performance liquid chromatography method employing diode array detection was developed to determine levels of the major catechins, proanthocyanidin (procyanidin B2), caffeine, thymoquinone and carvacrol and its isomer, thymol, which are present in different natural complex matrices found in commercial products of Camellia sinensis L. and/or Nigella sativa L. Reversed-phase separation was performed on a C18 column by using gradient elution by varying the proportions of solvent A (distilled water containing 0.05% orthophosphoric acid) and solvent B (acetonitrile), with a flow rate of 1.5 mL/min and duration of 31 min. Excellent linearity was observed for all standard calibration curves, and correlation coefficients were above 0.9996. The developed method is efficient, with high reproducibility and sensitivity, and is ideally suited for rapid and routine analysis of principal components in these promising medicinal plants.

PMID:
23811048
DOI:
10.1093/chromsci/bmt086
[PubMed - indexed for MEDLINE]
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