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J Clin Virol. 2013 Sep;58(1):283-5. doi: 10.1016/j.jcv.2013.05.021. Epub 2013 Jun 25.

Influenza virus A(H3N2) strain isolated from cerebrospinal fluid from a patient presenting myelopathy post infectious.

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1
Núcleo de Doenças Respiratórias, Instituto Adolfo Lutz, São Paulo, SP, Brazil. tterezinha@uol.com.br

Abstract

BACKGROUND:

Neurological involvement during influenza infection has been described during epidemics and is often consistent with serious sequelae or death.

OBJECTIVE:

To investigate the etiologic agent involved in myelopathy post influenza-like syndrome.

STUDY DESIGN:

This investigation focuses on virus isolation from the cerebrospinal fluid (CSF) collected from a 19-year-old male student presenting with clinical diagnosis of myelopathy post influenza-like syndrome. To achieve this goal, different cell cultures and molecular methodologies were carried out.

RESULTS:

Influenza virus A(H3N2) strain was isolated in MDCK cell culture; virus particles were observed under electron microscopy. Phylogenetics analyses showed that the Brazilian influenza A(H3N2) strains were closely related to the A/Perth/16/2009-like.

CONCLUSION:

This study demonstrates that influenza virus A(H3N2) strain was the cause of illness of the students. According to the Brazilian influenza virus sentinel surveillance data A/Perth/16/2009-LIKE (H3N2) strain has predominated during the 2010 influenza virus season in Brasília-DF.

KEYWORDS:

Cerebrospinal fluid; Influenza A(H3N2) strain; Laboratory diagnosis; Real time RT-PCR; Virus sequencing

PMID:
23809474
DOI:
10.1016/j.jcv.2013.05.021
[Indexed for MEDLINE]
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