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Methods Mol Biol. 2013;1036:163-78. doi: 10.1007/978-1-62703-511-8_14.

Decellularized whole heart for bioartificial heart.

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Department for Cardiovascular Surgery, Heinrich Heine University, Duesseldorf, Germany.


Whole-organ decellularization has opened the gates to the creation of 3D extracellular matrix (ECM) templates that mimic nature's design to a degree that-as for today-is not reproducible with any synthetic materials. Here, we describe a whole-heart decellularization approach through software-controlled automated coronary perfusion with standard decellularization detergents, enabling us to create native ECM-derived 3D templates that preserve the basic anatomy, vascular network, and critical ECM characteristics of the native heart. Such a cardiac ECM platform directly derived from nature itself might help us to better understand and reproduce cardiac biology and may even lay the grounds for the construction of a bioartificial heart in the future.

[Indexed for MEDLINE]

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