Format

Send to

Choose Destination
Channels (Austin). 2013 Jul-Aug;7(4):322-8. Epub 2013 Jun 27.

Molecular heterogeneity of large-conductance calcium-activated potassium channels in canine intracardiac ganglia.

Author information

1
Cardiovascular Genetics Center; Institut d'Investigació Biomèdica de Girona (IdIBGi); Department of Medical Sciences; School of Medicine; University of Girona (UdG); Girona, Spain.

Abstract

Large conductance calcium-activated potassium (BK) channels are widely expressed in the nervous system. We have recently shown that principal neurons from canine intracardiac ganglia (ICG) express a paxilline- and TEA-sensitive BK current, which increases neuronal excitability. In the present work, we further explore the molecular constituents of the BK current in canine ICG. We found that the β1 and β4 regulatory subunits are expressed in ICG. Single channel voltage-dependence at different calcium concentrations suggested that association of the BKα with a particular β subunit was not enough to explain the channel activity in this tissue. Indeed, we detected the presence of several splice variants of the BKα subunit. In conclusion, BK channels in canine ICG may result from the arrangement of different BKα splice variants, plus accessory β subunits. The particular combinations expressed in canine IC neurons likely rule the excitatory role of BK current in this tissue.

KEYWORDS:

BK channels; alternative splicing; autonomic; intracardiac neurons; β subunits

PMID:
23807090
PMCID:
PMC3989361
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center