Send to

Choose Destination
See comment in PubMed Commons below
J Chromatogr A. 2013 Aug 2;1301:156-61. doi: 10.1016/j.chroma.2013.05.076. Epub 2013 Jun 6.

Polyacrylamide brush layer for hydrophilic interaction liquid chromatography of intact glycoproteins.

Author information

  • 1Purdue University, Department of Chemistry, 560 Oval Drive, West Lafayette, IN 47907, USA.


A chromatographic column of nonporous silica particles with a bonded phase of linear polyacrylamide chains is evaluated for hydrophilic interaction liquid chromatography (HILIC) of intact glycoproteins. The column is shown to retain glycoproteins significantly more strongly than non-glycoproteins. A particle diameter of 700nm gives two-fold higher resolution than does a 1.4μm particle diameter, and the column efficiency is found to be mostly limited by packing heterogeneity. LCMS is able to resolve the five glycoforms of ribonuclease B and give high quality mass spectra, but there is loss of resolution of the isomers of glycoforms due to the lower amount of TFA. Compared to two leading commercial HILIC columns operated at 60°C, the polyacrylamide column operated at 30°C provided at least two-fold higher resolution for intact ribonuclease B, and showed peaks for glycoforms of prostate specific antigen, although not resolved.

Copyright © 2013 Elsevier B.V. All rights reserved.


Glycoprotein; HILIC

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk