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Cereb Cortex. 2014 Nov;24(11):3046-58. doi: 10.1093/cercor/bht161. Epub 2013 Jun 26.

Characterization and distribution of Reelin-positive interneuron subtypes in the rat barrel cortex.

Author information

1
Center for Neuroscience, Department of Neuroanatomy, Albert-Ludwigs-University, D79104 Freiburg, Germany Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75235, USA.
2
Center for Neuroscience, Department of Neuroanatomy, Albert-Ludwigs-University, D79104 Freiburg, Germany Department of Human Morphology and Developmental Biology, Semmelweis University, H1094 Budapest, Hungary.
3
Neurobiologie des Processus Adaptatifs, CNRS UMR7102, Université Pierre et Marie Curie, F75005 Paris, France.
4
Laboratoire de Neurobiologie, CNRS UMR7637, Ecole Supérieure de Physique et de Chimie Industrielles de Paris, F75005 Paris, France.
5
Center for Neuroscience, Department of Neuroanatomy, Albert-Ludwigs-University, D79104 Freiburg, Germany.
6
Center for Neuroscience, Department of Neuroanatomy, Albert-Ludwigs-University, D79104 Freiburg, Germany Clinic for Gastroenterology, Hepatology and Infectiology, Heinrich-Heine-University, D40225 Düsseldorf, Germany.
7
Center for Neuroscience, Department of Neuroanatomy, Albert-Ludwigs-University, D79104 Freiburg, Germany Institute for Structural Neurobiology, Center for Molecular Neurobiology, D20251 Hamburg, Germany and.
8
Center for Neuroscience, Department of Neuroanatomy, Albert-Ludwigs-University, D79104 Freiburg, Germany Institute for Neuroanatomy, UMG, Georg-August-University, D37075 Göttingen, Germany.

Abstract

GABAergic inhibitory interneurons (IN) represent a heterogeneous population with different electrophysiological, morphological, and molecular properties. The correct balance between interneuronal subtypes is important for brain function and is impaired in several neurological and psychiatric disorders. Here we show the data of 123 molecularly and electrophysiologically characterized neurons of juvenile rat barrel cortex acute slices, 48 of which expressed Reelin (Reln). Reln mRNA was exclusively detected in Gad65/67-positive cells but was found in interneuronal subtypes in different proportions: all cells of the adapting-Somatostatin (SST) cluster expressed Reln, whereas 63% of the adapting-neuropeptide Y (NPY, 50% of the fast-spiking Parvalbumin (PVALB), and 27% of the adapting/bursting-Vasoactive Intestinal Peptide (VIP) cluster were Reln-positive. Silhouette analysis revealed a high impact of the parameter Reln on cluster quality. By analyzing the co-localization of RELN immunoreactivity with those of different IN-markers, we found that RELN is produced layer-independently in SST-, NPY-, and NOS1-expressing INs, whereas co-localization of RELN and VIP was mostly absent. Of note, RELN co-localized with PVALB, predominantly in INs of layers IV/V (>30%). Our findings emphasize RELN's role as an important IN-marker protein and provide a basis for the functional characterization of Reln-expressing INs and its role in the regulation of inhibitory IN networks.

KEYWORDS:

clustering; glutamate decarboxylase; neuropeptide Y; polythetic classification; somatosensory cortex

PMID:
23803971
PMCID:
PMC4193467
DOI:
10.1093/cercor/bht161
[Indexed for MEDLINE]
Free PMC Article

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