Format

Send to

Choose Destination
See comment in PubMed Commons below
J Leukoc Biol. 2013 Sep;94(3):503-12. doi: 10.1189/jlb.0113057. Epub 2013 Jun 25.

FMNL1 promotes proliferation and migration of leukemia cells.

Author information

  • 1Department of Biological Sciences, Federal University of São Paulo, Diadema, São Paulo, Brazil. patricia.favaro@unifesp.br

Abstract

The human FMNL1 is expressed predominantly in hematopoietic cells and has been described previously as overexpressed in hematopoietic malignancies. However, it is not known whether FMNL1 contributes to leukemogenesis. Here, we investigate the FMNL1 function using two different human leukemia models: Namalwa and K562 cell lines. FMNL1 depletion reduced cell proliferation and colony formation in both leukemic cell types, as well as a decrease in the tumor growth of FMNL1-depleted Namalwa cell xenografts. In addition, there was a decrease in migration and in TEM in FMNL1-depleted Namalwa cells. FMNL1 endogenously associates with Rac1, and FMNL1 silencing resulted in an increased Rac1 activity. The reduced migration observed in FMNL1-depleted cells was restored by inhibiting Rac activity. Our results indicate that FMNL1 stimulates leukemia cell proliferation as well as migration. This suggests that FMNL1 contributes to leukemogenesis and could act in part through Rac1 regulation.

KEYWORDS:

Rac1; cell motility; formins; leukemogenesis; transendothelial migration

[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Write to the Help Desk