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Invest Ophthalmol Vis Sci. 2013 Jul 30;54(7):5103-10. doi: 10.1167/iovs.12-11491.

Effect of nitric oxide on anterior segment physiology in monkeys.

Author information

1
Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, Wisconsin, USA.

Abstract

PURPOSE:

To determine the effect of the nitric oxide donor, sodium nitroprusside (SNP), and the nitric oxide synthase (NOS) inhibitor, L-nitro-arginine-methylester (L-NAME), on IOP, mean arterial pressure (MAP), pupil diameter (PD), refraction (Rfx), aqueous humor formation (AHF), and outflow facility (OF) in monkeys.

METHODS:

Monkeys were treated with single or multiple topical treatments of 500 μg SNP or L-NAME to one eye. IOP was determined by Goldmann applanation tonometry, PD with vernier calipers in room light, Rfx by Hartinger coincidence refractometry, AHF by fluorophotometry, and MAP with a blood pressure monitor. OF was determined by two-level constant pressure perfusion following anterior chamber exchange.

RESULTS:

Following four topical treatments with 500 μg SNP, 30 minutes apart, IOP was significantly decreased from 2 to 6 hours compared with the contralateral control with the maximum IOP reduction of 20% at 3 hours (P < 0.001). PD, Rfx, and AHF were unchanged. Effects on MAP were variable. OF after SNP exchange was significantly increased by 77% (P < 0.05) at 10(-3) M. Topical L-NAME had no effect on IOP, PD, Rfx, or MAP.

CONCLUSIONS:

Enhancement of nitric oxide concentration at targeted tissues in the anterior segment may be a useful approach for IOP reduction for glaucoma therapy. Additional studies are warranted before conclusions can be made regarding the effect of NOS inhibition on ocular physiology in nonhuman primates.

KEYWORDS:

intraocular pressure; nitric oxide; outflow facility

PMID:
23800771
PMCID:
PMC3729238
DOI:
10.1167/iovs.12-11491
[Indexed for MEDLINE]
Free PMC Article
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