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J Lab Autom. 2014 Feb;19(1):50-9. doi: 10.1177/2211068213494390. Epub 2013 Jun 24.

Electrical impedance monitoring of photothermal porated mammalian cells.

Author information

1
1Precision and Intelligence Laboratory, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan.

Abstract

To transfer large cargo into mammalian cells, we recently provided a new approach called a photothermal nanoblade. Micron-sized membrane pores generated by the nanoblade are surprisingly well repaired with little cell death, suggesting rapid membrane-resealing dynamics. Here, we report the resealing time of photothermal porated mammalian cell plasma membranes using an electrical impedance sensor. Cell membrane pores were generated by high-speed cavitation bubbles induced by laser pulsing of metallic microdisks on a pair of transparent indium tin oxide electrodes. Electrical responses from the sensor electrodes were obtained with a signal voltage of 500 mV and a frequency at 500 kHz. Real-time impedance measurements show that membrane resealing and impedance recovery take a surprisingly long 1 to 2 min after laser pulsing. A nonrecovering impedance shift is also detected for cells after high-energy laser pulsing. This impedance response is also confirmed by a separate experiment in which thin-film gold electrodes are used to trigger cavitation bubbles for opening transient membrane pores on cells cultured on electrodes. Overall, our study platform provides new insight for micron-sized membrane defect repair dynamics to maintain cell viability.

KEYWORDS:

cell membrane; cell surgery; impedance sensor; membrane pores; photothermal effect; pulsed laser

PMID:
23797097
DOI:
10.1177/2211068213494390
[Indexed for MEDLINE]
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