Format

Send to

Choose Destination
J Sci Food Agric. 2013 Dec;93(15):3820-5. doi: 10.1002/jsfa.6287. Epub 2013 Jul 25.

Anti-inflammatory and immunomodulatory activities of stevioside and steviol on colonic epithelial cells.

Author information

1
Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand.

Abstract

BACKGROUND:

Stevioside is a natural non-caloric sweetener isolated from Stevia rebaudiana Bertoni leaves. We have proposed its effect on attenuation of tumour necrosis factor α (TNF-α) and interleukin 1β (IL-1β) release in lipopolysaccharide (LPS)-stimulated monocytes. In this study, the anti-inflammatory and immunomodulatory activities of stevioside and its metabolite, steviol, on human colon carcinoma cell line (Caco-2) were evaluated.

RESULTS:

Stevioside and steviol, in the doses used in this study, had no cytotoxicity on Caco-2 cells. Anti-inflammatory activities of these two compounds were observed by potentially suppressed LPS-mediated TNF-α, IL-1β and IL-6 release. In addition, stevioside and steviol showed immunomodulatory effects on IκBα activation and nuclear factor kappa B (NF-κB) suppression in western blotting.

CONCLUSION:

Stevioside and steviol attenuate LPS-induced pro-inflammatory cytokine productions by affecting cytokine gene expression via IκBα/NF-κB signalling pathway.

KEYWORDS:

Caco-2 cells; NF-κB; inflammatory cytokine; steviol; stevioside

PMID:
23794454
DOI:
10.1002/jsfa.6287
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center