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Nat Methods. 2013 Aug;10(8):744-6. doi: 10.1038/nmeth.2528. Epub 2013 Jun 23.

Multiplexed MS/MS for improved data-independent acquisition.

Author information

1
Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington, USA.

Abstract

In mass spectrometry-based proteomics, data-independent acquisition (DIA) strategies can acquire a single data set useful for both identification and quantification of detectable peptides in a complex mixture. However, DIA data are noisy owing to a typical five- to tenfold reduction in precursor selectivity compared to data obtained with data-dependent acquisition or selected reaction monitoring. We demonstrate a multiplexing strategy, MSX, for DIA analysis that increases precursor selectivity fivefold.

PMID:
23793237
PMCID:
PMC3881977
DOI:
10.1038/nmeth.2528
[Indexed for MEDLINE]
Free PMC Article

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