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Bioresour Technol. 2013 Sep;143:104-11. doi: 10.1016/j.biortech.2013.05.122. Epub 2013 Jun 6.

Evaluation of integrated anaerobic-aerobic biofilm reactor for degradation of azo dye methyl orange.

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School of Environmental Engineering, Universiti Malaysia Perlis, 02600 Arau, Perlis, Malaysia.


This study was to investigate the mineralization of wastewater containing methyl orange (MO) in integrated anaerobic-aerobic biofilm reactor with coconut fiber as bio-material. Different aeration periods (3h in phase 1 and 2; 3, 6 and 15 h in phase 3; 24 h in phase 4 and 5) in aerobic chamber were studied with different MO concentration 50, 100, 200, 200 and 300 mg/L as influent from phase 1-5. The color removals estimated from the standard curve of dye versus optical density at its maximum absorption wavelength were 97%, 96%, 97%, 97%, and 96% and COD removals were 75%, 72%, 63%, 81%, and 73% in phase 1-5, respectively. The MO decolorization and COD degradation followed first-order kinetic model and second-order kinetic model, respectively. GC-MS analysis indicated the symmetrical cleavage of azo bond and the reduction in aromatic peak ensured the partial mineralization of MO.


Biofilm; Color removal; Integrated anaerobic–aerobic; Kinetic model; Methyl orange

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