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Biochem Biophys Res Commun. 2013 Jul 12;436(4):719-24. doi: 10.1016/j.bbrc.2013.06.022. Epub 2013 Jun 17.

An attenuating role of a WASP-related protein, WASP-B, in the regulation of F-actin polymerization and pseudopod formation via the regulation of RacC during Dictyostelium chemotaxis.

Author information

1
Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN, USA. chang.chung@vanderbilt.edu

Abstract

The WASP family of proteins has emerged as important regulators that connect multiple signaling pathways to regulate the actin cytoskeleton. Dictyostelium cells express WASP, as well as a WASP related protein, WASP-B, endoded by wasB gene. WASP-B contains many of the domains present in WASP. Analysis of wild type, wasB null cells revealed that WASP-B is required for proper control of F-actin polymerization in response to a cAMP gradient. Due to the lack of tight control on actin polymerization, wasB null cells exhibited higher level of F-actin polymerization. wasB(-) cells extend more de novo pseudopods laterally and their average life span is longer than those of wild type cells, causing more turns and inefficient chemotaxis. YFP-WASP-B appears to be uniformly distributed in the cytosol and shows no translocation to cortical membrane upon cAMP stimulation. Active RacC pull-down assay reveals that the level of active RacC in wasB(-) cells is significantly higher than wild type cells. Moreover, the distribution of active RacC is not localized in wasB(-) cells. We conclude that chemotaxis defects of wasB(-) cells are likely to result from the aberrant regulation of RacC activation and localization.

KEYWORDS:

Chemotaxis; Dictyostelium; F-actin; WASP

PMID:
23791739
PMCID:
PMC3799868
DOI:
10.1016/j.bbrc.2013.06.022
[Indexed for MEDLINE]
Free PMC Article
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