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Cell Stem Cell. 2013 Aug 1;13(2):190-204. doi: 10.1016/j.stem.2013.05.015. Epub 2013 Jun 20.

In vivo mapping of notch pathway activity in normal and stress hematopoiesis.

Author information

1
Howard Hughes Medical Institute and NYU Cancer Institute, New York University School of Medicine, New York, NY 10016, USA.
2
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
3
Department of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Stem Cell Research Center, University of California Los Angeles, 621 Charles E. Young Drive S., Los Angeles, CA 90095, USA.
4
Department of Pathology and NYU Cancer Institute, New York University School of Medicine, New York, NY 10016, USA.
5
Exelixis Inc, South San Francisco, CA 94080, USA.
6
Collège de France, 75231 Paris Cedex 05 France.
#
Contributed equally

Erratum in

  • Cell Stem Cell. 2013 Aug 1;13(2):256. Manet, Jan [corrected to Manent, Jan].

Abstract

Accumulating evidence suggests that Notch signaling is active at multiple points during hematopoiesis. Until recently, the majority of such studies focused on Notch signaling in lymphocyte differentiation and knowledge of individual Notch receptor roles has been limited due to a paucity of genetic tools available. In this manuscript we generate and describe animal models to identify and fate-map stem and progenitor cells expressing each Notch receptor, delineate Notch pathway activation, and perform in vivo gain- and loss-of-function studies dissecting Notch signaling in early hematopoiesis. These models provide comprehensive genetic maps of lineage-specific Notch receptor expression and activation in hematopoietic stem and progenitor cells. Moreover, they establish a previously unknown role for Notch signaling in the commitment of blood progenitors toward the erythrocytic lineage and link Notch signaling to optimal organismal response to stress erythropoiesis.

PMID:
23791481
PMCID:
PMC3902172
DOI:
10.1016/j.stem.2013.05.015
[Indexed for MEDLINE]
Free PMC Article

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