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Adv Healthc Mater. 2014 Jan;3(1):42-6. doi: 10.1002/adhm.201300056. Epub 2013 Jun 21.

A reusable sensor for the label-free detection of specific oligonucleotides by surface plasmon fluorescence spectroscopy.

Author information

1
Nöll Junior Research Group for Nanotechnology, Siegen University, Faculty IV, Department of Chemistry-Biology, Organic Chemistry, Adolf-Reichwein-Str. 2, 57068 Siegen, Germany. noell@chemie.uni-siegen.de.

Abstract

The development of a reusable molecular beacon (MB)-based sensor for the label-free detection of specific oligonucleotides using surface plasmon fluorescence spectroscopy (SPFS) as the readout method is described. The MBs are chemisorbed at planar gold surfaces serving as fluorescence quenching units. Target oligonucleotides of 24 bases can be detected within a few minutes at high single-mismatch discrimination rates.

KEYWORDS:

DNA; molecular beacon (MB); oligonucleotides; single-nucleotide polymorphism; surface plasmon fluorescence spectroscopy (SPFS)

PMID:
23788367
DOI:
10.1002/adhm.201300056
[Indexed for MEDLINE]

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